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No. 21), whereby 50 and 501 bp hG dsRNA corresponded to positions 121 and 121 respectively, to the start codon.For annealing of siRNAs, 20 M single strands were incubated in annealing buffer (100 mM potassium acetate, 30 mM HEPES KOH at pH 7.4, 2 mM magnesium acetate) for 1 min at 90 followed by 1 h at 37 The 37 incubation step was extended overnight for the 50 and 500 bp dsRNAs, and these annealing reactions were performed at 8.4 M and 0.84 M strand concentrations, respectively.Cell culture S2 cells were propagated in Schneider's Drosophila medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS) 100 units ml 1 penicillin, and 100 g ml 1 streptomycin at 25 293, NIH/3T3, HeLa S3, HeLa SS6, COS 7 cells were grown at 37 in Dulbecco's modified Eagle's medium supplemented with 10% FBS, 100 units ml 1 penicillin, and 100 g ml 1 streptomycin.
results of Vice President Joe Biden's task force on gun violence prevention by releasing a controversial ad that targets the president's family. Amid a widespread backlash, White House press secretary Jay Carney offered a statement: "Most Americans agree that a president's children should not be used as pawns in a political fight.
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